Journal: PLoS ONE

Article Title: Decrease in Blood Pressure and Regression of Cardiovascular Complications by Angiotensin II Vaccine in Mice

doi: 10.1371/journal.pone.0060493

Figure Lengend Snippet: (A) T cell proliferation was determined by analyzing [ 3 H]-thymidine incorporation. Splenocytes from mice on day 42 were stimulated for 36 hours with Ang II-KLH, KLH, Ang II or angiotensinogen (AGT) at a concentration of 10 µg/ml. The stimulation index is expressed as the ratio of stimulation to no stimulation. The data are expressed as the mean stimulation index ± the standard error of the mean per 10 6 splenocytes. * P <0.001 vs. no stimulation. (B) A representative photograph from the ELISPOT assay. The ELISPOT assay detected splenocytes that produced IL-4 and/or IFN-γ? Splenocytes from mice on day 42 were stimulated for 48 hours with 10 µg/ml Ang II-KLH, KLH, Ang II or angiotensinogen (AGT). (C) The quantification of spots in the ELISPOT assay. The data are expressed as the mean number of spots ± SEM per 10 6 splenocytes. * P <0.001 vs. saline. (a,c) The results are from 6 control mice (saline) and 6 experimental mice (1,000 ng Ang II-KLH with adjuvant).

Article Snippet: Briefly, 96-well ELISPOT plates (Millipore, Tokyo, Japan) were coated with anti-mouse IFN-γ or IL-4 antibody, as recommended by the manufacturer (R&D Systems, Tokyo, Japan).

Techniques: Concentration Assay, Enzyme-linked Immunospot, Produced, Saline, Adjuvant

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Primary alloproliferative TH1 response induced by immature plasmacytoid dendritic cells in collaboration with myeloid DCs.

doi: 10.1111/j.1600-6143.2005.01097.x

Figure Lengend Snippet: Figure 4: The phenotype of the responder T-cell population is similar in MDC and 5000PDC+100MDC allostimulatory conditions. After 5 days of culture, the alloproliferating CFSE-low T cells were stained with different mAbs to the indicated markers. (A) The expression of IL2Ra, IL12Rb1 and CD154 on proliferating T cells (gated on R1) is shown. The numbers indicate the percentage of cells in each quadrant. (B) Summary of the markers analyzed and detected (+ve) on responding CFSE-low T cells stimulated by 5000MDC (black bars) or 5000PDC+100MDC (white bars) (mean ± SD, n = 4). As most cells expressing the markers are CFSE low, the proportion is calculated based on the proliferating cells (contained in the R2 regions). (C) The time-dependent expression of IL12Rb2 on CFSE-low T cells was analyzed in three different culture experiments stimulated by (5000PDC+100MDC) (white symbols) or 5000MDCs alone (black symbols) for the indicated time period.

Article Snippet: The following murine monoclonal antibodies (mAbs) were used: Peridinin Chlorophyll Protein (PerCP)-labeled CD3 and HLA-DR; Fluorescein isothiocyanate (FITC)-labeled CD4 and CD45RA; Phycoerythrin (PE)-labeled CD11c, CD123, IL2Ra, CD154, CD45RO, IL12Rb1, IL12Rb2, IL18Ra (BD Biosciences Oxford, UK); TRI-Color® (TC)-labeled CD3, PE-labeled IL4, Allophycocyanin (APC)-labeled IFNc (Caltag Laboratories, Hamburg, Germany); FITC-labeled anti-BDCA2 (Miltenyi Biotec, Bergisch Gladbach, Germany); RPE-Cy5-labeled CD14, CD19 (Serotec Ltd, Kidlington, Oxford, UK).

Techniques: Staining, Expressing